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Methods. 2001 Nov;25(3):303-8.

Construction of low-resolution x-ray crystallographic electron density maps of the ribosome.

Author information

1
Departments of Chemistry and Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA. jcate@lbl.gov

Abstract

Advances in X-ray crystallography now allow biological macromolecules of almost any size to be imaged at atomic resolution. Here, I outline the strategy that allowed for the solution of the 70S ribosome structure to 7.8-A resolution. The most important factors involve the effective use of synchrotron radiation and the application of existing crystallographic software to very large structures.

PMID:
11860284
DOI:
10.1006/meth.2001.1242
[Indexed for MEDLINE]
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