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Science. 2002 Feb 22;295(5559):1503-6.

Array-based electrical detection of DNA with nanoparticle probes.

Author information

1
Department of Chemistry and Institute for Nanotechnology, Northwestern University, Evanston, IL 60208, USA.

Abstract

A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of approximately 100,000:1.

PMID:
11859188
DOI:
10.1126/science.1067003
[Indexed for MEDLINE]
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