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Diagn Microbiol Infect Dis. 2002 Feb;42(2):99-105.

Rapid diagnosis of enterovirus infections by real-time PCR on the LightCycler using the TaqMan format.

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1
The Division of Clinical Virology, Institute of Virology and Infectious Diseases, University Hospital Vienna, A-1090, Vienna, Austria. thomas.watkins-riedel@akh-wien.ac.at

Abstract

A 2 h single-tube, reverse-transcription(RT)-PCR/hybridization assay using the TaqMan format for rapid diagnostic screening of enterovirus (EV) infections was optimized for the real-time LightCycler (LC) technology. For low EV load clinical samples an additional 30 min reamplification step using a novel primer-mix/probe design resulted in a 100% concordance with AMPLICOR EV PCR Test and in-house RT-PCR. Combined with maximum specificity, the sensitivity of LC-PCR was 10- to 100-fold higher compared to AMPLICOR EV Test.

PMID:
11858904
[Indexed for MEDLINE]

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