Format

Send to

Choose Destination
Curr Eye Res. 2001 Aug;23(2):116-9.

Proliferation in the posterior region of the lens of c-maf-/- mice.

Author information

1
Department of Ophthalmology, Hokkaido University School of Medicine, Kita-ku, Sapporo, Japan. kyoshida@med.hokudai.ac.jp

Abstract

PURPOSE:

To examine the involvement of the c-maf gene in the proliferation of the lens cells.

METHODS:

Eyes of the E13 and E18 stages of the wild-type and c-maf-/- mice were analyzed by BrdU incorporation assay, TUNEL assay and immunocytochemistry using a anti-P27(KIP1) and a anti-P57(KIP2) antibody.

RESULTS:

In the E13 and E18 c-maf mutant lens, BrdU-positive cells were detected at the posterior region of the lens. Cell-cycle inhibitor P27(KIP1) and P57(KIP2) were expressed in the equatorial and posterior region of the lens of both wild-type and c-maf-/- lenses.

CONCLUSION:

These results suggest that the expression of c-maf is required for differentiation and cell cycle arrest of lens fiber cells. It is also suggested that P27(KIP1) and P57(KIP2) were not involved in the continued proliferation of posterior region of the c-maf-/- lens.

PMID:
11840349
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center