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Arterioscler Thromb Vasc Biol. 2002 Feb 1;22(2):329-34.

Platelet shape changes and adhesion under high shear flow.

Author information

1
Department of Pediatrics, Nara Medical University, Kashihara, Nara, Japan.

Abstract

Recent studies have revealed that the platelet adhesive process under flow is tightly regulated by multiple ligand-receptor interactions. However, platelet morphological changes during this process, particularly its physiological relevance, remain unknown under blood flow conditions. Using epifluorescence and scanning electron microscopy, we evaluated the real-time changes in platelet morphology during a platelet adhesive process on a von Willebrand factor-coated surface under physiological high shear flow in a perfusion chamber. Here, we show that dynamic platelet shape changes occurring during distinct phases of the adhesive process are precisely regulated by "inside-out" and "outside-in" integrin signals and are also a key regulatory element in successful platelet thrombogenesis opposing rapid blood flow in vivo.

PMID:
11834537
[Indexed for MEDLINE]

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