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J Chromatogr A. 2002 Jan 18;943(2):299-302.

Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography.

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1
Institute of Food Chemistry, Technical University of Braunschweig, Germany.

Abstract

High-speed counter-current chromatography was successfully used for the isolation and purification of secoisolariciresinol diglucoside, a bioactive lignan from flaxseed (Linum usitatissimum L.). The solvent system consisted of tert.-butylmethyl ether-n-butanol-acetonitrile-water (1:3:1:5). The purity and identity of the isolated compound was checked by high-performance liquid chromatography analysis in combination with mass spectrometry and NMR measurements.

PMID:
11833650
[Indexed for MEDLINE]
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