Ncad/GFP-containing vesicular structures associate with MTs. Ncad/GFP-expressing cells were fixed and processed for immunofluorescence by using rhodamine-labeled phalloidin and anti-vimentin or anti-α-tubulin antibodies followed by rhodamin-labeled secondary antibodies. Stacks of images were acquired using a wide-field microscope (0.1 μm Z step) and deconvolved using the Huygens System image restoration software. Deconvolved stacks were visualized using the Iview command of the Imaris software (a, c, and e). a, c, and e show the Ncad/GFP (in green), F-actin (a, red), vimentin IF (c, red), and α-tubulin staining (e, red). Respective voxel colocalizations of Ncad/GFP, with F-actin, vimentin, and tubulin obtained using the Imaris Colocalization module are shown in b, d, and f. A single confocal section of Ncad/GFP-expressing cells (green) stained with an anti-α-tubulin antibody (red) is shown in g. h illustrates in white the colocalized objects in the selected area in g. Bar, 10 μm.