The effect soft tissue movement of the shank had on knee joint moments during natural cadence walking was investigated in this study. This was examined by comparing knee moments determined from bone-anchored and surface mounted tracking targets. Six healthy adult subjects participated in this study. The largest difference (3 N m) occurred about the AP axis, with smaller differences of approximately 2 and 1 N m about the flexion/extension (F/E) and longitudinal (Long) axes, respectively. The magnitude of these differences would not likely affect the clinical interpretation of the data. The effect of reporting knee moments in two different orthogonal reference systems was also examined. The peak extension moment was significantly greater when expressed about an anatomical axis following the line of the malleoli than when the moment was reported about an axis parallel to the frontal plane of the shank. In contrast, the first peak abduction moment was significantly greater when expressed about an axis perpendicular to the frontal plane of the shank. Care should therefore be exercised whenever comparisons between studies are made in which the reference axes are not aligned.