Abstract
Generation of CTL immunity often depends on the availability of CD4 T cell help. In this report, we show that CTL responses induced by cross-priming can be converted from CD4-dependent to CD4-independent by increasing the frequency of CTL precursors. In the absence of CD4 T cells, high numbers of CTL precursors were able to expand in number and become effector CTL. The ability of high frequencies of CD8 T cells to override help was not due to their ability to signal CD40 via expression of CD154. These findings suggest that when precursor frequencies are high, priming of CD8 T cell responses may not require CD4 T cell help.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens / pharmacology
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CD40 Ligand / biosynthesis
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CD40 Ligand / physiology
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Epitopes, T-Lymphocyte / administration & dosage
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Epitopes, T-Lymphocyte / immunology
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Histocompatibility Antigens Class II / genetics
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Injections, Intravenous
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Lymphocyte Activation / genetics
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Lymphocyte Count
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Ovalbumin / administration & dosage
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Ovalbumin / genetics
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Ovalbumin / immunology
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Spleen / cytology
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Spleen / immunology
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Spleen / transplantation
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Stem Cells / cytology*
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Stem Cells / immunology*
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T-Lymphocytes, Cytotoxic / cytology*
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T-Lymphocytes, Cytotoxic / immunology*
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T-Lymphocytes, Cytotoxic / transplantation
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T-Lymphocytes, Helper-Inducer / immunology*
Substances
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Antigens
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Epitopes, T-Lymphocyte
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Histocompatibility Antigens Class II
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CD40 Ligand
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Ovalbumin