Format

Send to

Choose Destination
See comment in PubMed Commons below
Genes Dev. 2002 Jan 15;16(2):209-21.

kuzbanian-mediated cleavage of Drosophila Notch.

Author information

1
Laboratory of Genetics, The Rockefeller University, New York, NY 10021, USA.

Abstract

Loss of Kuzbanian, a member of the ADAM family of metalloproteases, produces neurogenic phenotypes in Drosophila. It has been suggested that this results from a requirement for kuzbanian-mediated cleavage of the Notch ligand Delta. Using transgenic Drosophila expressing transmembrane Notch proteins, we show that kuzbanian, independent of any role in Delta processing, is required for the cleavage of Notch. We show that Kuzbanian can physically associate with Notch and that removal of kuzbanian activity by RNA-mediated interference in Drosophila tissue culture cells eliminates processing of ligand-independent transmembrane Notch molecules. Our data suggest that in Drosophila, kuzbanian can mediate S2 cleavage of Notch.

PMID:
11799064
PMCID:
PMC155326
DOI:
10.1101/gad.942302
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center