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Arch Biochem Biophys. 2002 Jan 15;397(2):258-61.

CAAT/enhancer binding protein activates an enhancer in the glutamine synthetase distal 5'-flanking sequence.

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  • 1Research Service, The John D. Dingell VA Medical Center, 4646 John R., Detroit, Michigan 48201, USA. thadden@cmb.biosci.wayne.edu

Abstract

The glutamine synthetase (GS) gene is expressed at high levels in several cell types, including astrocytes, pericentral hepatocytes, and adipocytes. During hormone-mediated adipocyte differentiation of 3T3-L1 cells, GS gene expression increases several hundred fold. We previously reported that elements in the distal 5'-flanking sequence and intron-1 participate in establishing the temporal pattern of GS transcription during adipocyte differentiation. To examine the role of the distal 5'-flanking region in regulating adipocyte-specific GS expression, GS-CAT fusion genes were constructed and analyzed in transiently transfected 3T3-L1 cells. In this way, adipocyte differentiation-responsive enhancer activity was localized to a 422-bp sequence that occurs about 3.5 kb upstream from the transcription start site. This sequence includes several putative C/EBP binding sites and is activated by ectopic expression of C/EBPalpha in NIH-3T3 cells. Thus, our data indicate that C/EBPalpha has the capacity to activate functional C/EBP sites in the GS gene distal 5'-flanking region.

PMID:
11795880
DOI:
10.1006/abbi.2001.2666
[PubMed - indexed for MEDLINE]
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