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J Physiol. 2002 Jan 15;538(Pt 2):383-9.

Enhancement of presynaptic calcium current by cysteine string protein.

Author information

1
Synaptic Mechanisms Section, NINDS, NIH, Bethesda, MD 20892-4156, USA.

Abstract

The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic N-type Ca(2+) channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca(2+) current amplitude. However, this increase could not be attributed to a change in Ca(2+) channel kinetics, voltage dependence, prepulse inactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Secretory vesicle associated endogenous CSP may play an important role in enhancing Ca(2+) channel activity at the transmitter release site.

PMID:
11790807
PMCID:
PMC2290073
DOI:
10.1113/jphysiol.2001.013397
[Indexed for MEDLINE]
Free PMC Article

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