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Chin Med J (Engl). 2001 Aug;114(8):833-6.

The role of hypertension-related gene in aortic vascular smooth muscle cells from mice and rats.

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1
Institute of Cardiovascular Research, Beijing Medical University, Beijing 100083, China.

Abstract

OBJECTIVE:

To study the role of hypertension-related gene (HRG-1) in cardiovascular disease.

METHODS:

The expression of HRG-1 was analyzed with RT-PCR and Northern blotting. Vascular smooth muscle cell (VSMC) proliferation was measured with 3H-TdR incorporation and was confirmed with histological analysis.

RESULTS:

Northern blot analysis showed that HRG-1 mRNA was expressed not only in VSMC, but also in various rat tissues (heart, brain, lung, kidney, and liver). In addition, the expression of HRG-1 mRNA in heart, brain, kidney and liver of spontaneously hypertensive rat (SHR) was lower than that in the same tissues of Wistar-Kyotorat (WKY). Semi-quantitative RT-PCR and histological analysis showed that the expression of HRG-1 mRNA in ApoE-knockout mice and in animal models of restenosis was decreased and neointimal formation was observed in both models. ET, AII, and IL-1 stimulating VSMC proliferation reduced the expression of HRG-1 mRNA of VSMC. Atrial natriuretic factor (ANF), calcitonin gene-related peptide and adrenomedullin, inhibited VSMC proliferation and elevated the expression of HRG-1 mRNA. These effects could be blocked or attenuated by their corresponding antagonists or antibodies.

CONCLUSION:

HRG-1 is a gene related to VSMC proliferation. It may play an important role in several occlusive cardiovascular diseases including atherosclerosis, restenosis and hypertension.

PMID:
11780361
[Indexed for MEDLINE]

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