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Fertil Steril. 2002 Jan;77(1):152-8.

Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes.

Author information

1
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.

Abstract

OBJECTIVE:

To determine the effectiveness of trehalose as an intracellular cryoprotectant for the cryopreservation of human oocytes.

DESIGN:

In vitro comparative study.

SETTING:

Clinical and academic research environment at a medical school teaching hospital.

PATIENT(S):

Women undergoing in vitro fertilization (IVF).

INTERVENTION(S):

Discarded human oocytes, obtained from IVF patients, were randomly distributed into three groups: control group (no trehalose), extracellular trehalose group (0.5 M extracellular trehalose), and intracellular trehalose group (0.15 M intra- and 0.5 M extracellular trehalose). Trehalose was introduced into oocytes by microinjection. The oocytes in each group were cooled to different temperatures (i.e., -15 degrees C, -30 degrees C, and -60 degrees C) at rate of 1 degrees C/minute and thawed at ambient air temperature. Survival was examined after overnight culture.

MAIN OUTCOME MEASURE(S):

Survival of human oocytes cryopreserved in the presence and absence of trehalose.

RESULT(S):

The majority of oocytes in the intracellular trehalose group survived cooling to -15 degrees C (63%), -30 degrees C (53%), and -60 degrees C (66%). In contrast, only a small number of oocytes in both the control (13%) and extracellular trehalose group (22%) survived cooling to -15 degrees C, while all oocytes degenerated when cooled to -30 degrees C and -60 degrees C.

CONCLUSION(S):

Small amounts of intracellular trehalose in the absence of any other cryoprotectant provide a significant protection against freeze-associated stresses. Our results suggest that sugars such as trehalose should be considered as intracellular cryoprotectants for cryopreservation of human oocytes.

PMID:
11779606
[Indexed for MEDLINE]

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