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Anal Chem. 2001 Dec 1;73(23):5725-31.

Tandem mass spectrometry of intact proteins for characterization of biomarkers from Bacillus cereus T spores.

Author information

1
Department of Chemistry, University of Maryland, College Park 20742, USA. demirev@wam.umd.edu

Abstract

Intact protein biomarkers from Bacillus cereus T spores have been analyzed by high-resolution tandem Fourier transform ion cyclotron resonance mass spectrometry. Two techniques have been applied for excitation of the isolated multiply charged precursor ion species: sustained off-resonance irradiation/collisionally activated dissociation and electron capture dissociation. Fragmentation-derived sequence tags and BLAST sequence similarity proteome database searches allow unequivocal identification of the major biomarker protein with unprecedented specificity. Sequence-specific fragmentation patterns further confirm protein identification. Moreover, methodology combining accurate mass measurements of intact proteins with additional information contained in a proteome database permits tentative assignment of several other protein biomarkers isolated from the B. cereus T spores. We argue that approaches involving tandem MS of protein biomarkers, combined with bioinformatics, can drastically improve the specificity of individual microorganism identification, particularly in complex environments.

PMID:
11774913
DOI:
10.1021/ac010672n
[Indexed for MEDLINE]

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