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Bioinformatics. 2001 Dec;17(12):1226-7.

MELTING, computing the melting temperature of nucleic acid duplex.

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Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.


MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix-coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. Perl scripts are provided to show how MELTING can be used to construct more ambitious programs.


MELTING is available for several platforms ( and is accessible via a www server (


[Indexed for MEDLINE]

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