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FEMS Microbiol Lett. 2001 Dec 18;205(2):323-8.

Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced.

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Microbial Physiology Research Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, The Netherlands.


Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme produced a levan that is identical to that produced by Lb. reuteri strain 121 cells.

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