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Acta Pharmacol Sin. 2001 Sep;22(9):793-6.

Effect of genistein and quercetin on proliferation, collagen synthesis, and type I procollagen mRNA levels of rat hepatic stellate cells.

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1
Department of Pharmacy, Fuzhou General Hospital, Fuzhou 350001, China.

Abstract

AIM:

To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells.

METHODS:

Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by [3H]proline incorporation assay. Type I mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS:

GE (25 - 70 micromol/L) and QU (6.25 - 50 micromol/L) concentration-dependently reduced serum-driven HSC-T6 cell proliferation and collagen synthesis associated with a suppression of type I procollagen mRNA level.

CONCLUSION:

GE and QU inhibited hepatic stellate cell proliferation and collagen synthesis that might have a protective role against liver fibrosis.

PMID:
11749858
[Indexed for MEDLINE]
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