Send to

Choose Destination
Gynecol Oncol. 2002 Jan;84(1):126-34.

Activated matrix metalloproteinase-2--a potential marker of prognosis for epithelial ovarian cancer.

Author information

Department of Obstetrics and Gynecology, Fourth Affilaited Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050011, People's Republic of China.



To investigate the relationship between the expression of matrix metalloproteinase-2 (MMP-2) and clinical characteristics in patients with epithelial ovarian tumors, we examined the expression of MMP-2 in 26 epithelial benign ovarian tumors (EBOT) and 41 epithelial ovarian carcinomas (EOC) using semi-quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry. We also analyzed pro-MMP-2 and activated MMP-2 in epithelial ovarian tumors using Western blot. RESULTS The expression levels of MMP-2 mRNA and overall protein were higher in EOC than in EBOT, but the differences were not statistically significant (P > 0.05). MMP-2 mRNA and immunoreactive protein for MMP-2 were not significantly associated with clinicopathological features in EOC. The positive percentages of the active form of MMP-2 were 71% in EOC and 42% in EBOT, respectively (P < 0.05). The positive percentage of the active form of MMP-2 in stage III and IV EOC was significantly higher (81%) than that (33%) in stage I and II EOC (P = 0.01). The expression of activated MMP-2 was significantly related to disease progression in EOC (P = 0.02). The percentages of active MMP-2 in positive immunoreaction tumor cells and fibroblasts were, respectively, 96 and 89%. The difference was not statistically significant (P = 0.54). The positive and negative predictive values of active MMP-2 for disease progression were 65 (19/29) and 75% (9/12), respectively, and the accuracy was 68% (28/41).


MMP-2 generally appears in epithelial ovarian tumors and there is a tendency to express more MMP-2, and especially activated MMP-2, in EOC. MMP-2 mRNA and pro-MMP-2 are not associated with the clinicopathological features in patients with EOC. There was a significant relationship between activated MMP-2 and invasiveness, metastasis, and disease progression in EOC and activated MMP-2 is a potential marker of prognosis.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center