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Nat Genet. 2002 Jan;30(1):59-65. Epub 2001 Dec 17.

Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing.

Author information

1
Integrative Morphology Group, Department of Anatomy, University of Vienna, Waehringerstrasse 13 A-1090 Wien, Austria. Wolfgang.Weninger@univie.ac.at

Abstract

We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.

PMID:
11743576
DOI:
10.1038/ng785
[Indexed for MEDLINE]

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