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EMBO J. 2001 Dec 17;20(24):7294-302.

Nucleosome positioning at the replication fork.

Author information

1
Institute of Cell Biology, ETH Hönggerberg, CH-8093 Zürich, Switzerland.

Abstract

In order to determine the time required for nucleosomes assembled on the daughter strands of replication forks to assume favoured positions with respect to DNA sequence, psoralen cross-linked replication intermediates purified from preparative two-dimensional agarose gels were analysed by exonuclease digestion or primer extension. Analysis of sites of psoralen intercalation revealed that nucleosomes in the yeast Saccharomyces cerevisiae rDNA intergenic spacer are positioned shortly after passage of the replication machinery. Therefore, both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands, suggesting that the positioning of nucleosomes is an initial step in the chromatin maturation process.

PMID:
11743005
PMCID:
PMC125336
DOI:
10.1093/emboj/20.24.7294
[Indexed for MEDLINE]
Free PMC Article
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