Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2001 Dec 21;289(5):1237-42.

Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog.

Author information

1
Department of Microbiology, Chungnam National University, Taejon, 305-764, Korea.

Abstract

We have isolated a full-length cDNA clone that encodes for a Schizosaccharomyces pombe homolog of the dual-specificity protein kinase of the LAMMER family, lkh1 (lammer kinase homolog). The proposed Lkh1 protein contains 575 amino acids. The lkh1(+) null mutant is viable, but exhibits flocculation upon reaching stationary phase in liquid media and filamentous adhesion growth on solid media. Analysis of the flocculation activity of the lkh1(+) null mutant indicates that asexual aggregation of S. pombe cells into floccules is divalent cation-dependent and galactose-specific. We also demonstrate that the Saccharomyces cerevisiae LAMMER kinase homolog, Kns1, can substitute for the Lkh1 function in S. pombe.

PMID:
11741326
DOI:
10.1006/bbrc.2001.6128
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center