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Dev Cell. 2001 Dec;1(6):807-16.

neuralized Encodes a peripheral membrane protein involved in delta signaling and endocytosis.

Author information

1
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.

Abstract

Activation of the Notch (N) receptor involves an intracellular proteolytic step triggered by shedding of the extracellular N domain (N-EC) upon ligand interaction. The ligand Dl has been proposed to effect this N-EC shedding by transendocytosing the latter into the signal-emitting cell. We find that Dl endocytosis and N signaling are greatly stimulated by expression of neuralized (neur). neur inactivation suppresses Dl endocytosis, while its overexpression enhances Dl endocytosis and Notch-dependent signaling. We show that neur encodes an intracellular peripheral membrane protein. Its C-terminal RING domain is necessary for Dl accumulation in endosomes, but may be dispensable for Dl signaling. The potent modulatory effect of Neur on Dl activity makes Neur a candidate for establishing signaling asymmetries within cellular equivalence groups.

PMID:
11740942
DOI:
10.1016/s1534-5807(01)00093-4
[Indexed for MEDLINE]
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