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Pediatr Infect Dis J. 2001 Dec;20(12):1181-2.

Broad range polymerase chain reaction for diagnosis of rat-bite fever caused by Streptobacillus moniliformis.

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Division of Infectious Diseases, University Children's Hospital, Zurich, Switzerland.


An 11-year-old boy presented with fever, vomiting, rash, limping and blisters on his feet after a finger bite by his domestic rat. Although cultures from blood, cerebrospinal fluid and urine remained negative, broad range polymerase chain reaction amplification of a part of the 16S rRNA gene followed by sequencing allowed the detection and identification of Streptobacillus moniliformis in blister fluid, thus confirming the suspected clinical diagnosis of rat-bite fever.

[Indexed for MEDLINE]

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