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Biol Cell. 2001 Sep;93(1-2):27-33.

The pathway of MAP kinase mediation of CSF arrest in Xenopus oocytes.

Author information

1
Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver 80262, USA. Jim.Maller@uchsc.edu

Abstract

A cytoplasmic activity in mature oocytes responsible for second meiotic metaphase arrest was identified over 30 years ago in amphibian oocytes. In Xenopus oocytes CSF activity is initiated by the progesterone-dependent synthesis of Mos, a MAPK kinase kinase, which activates the MAPK pathway. CSF arrest is mediated by a sole MAPK target, the protein kinase p90Rsk which leads to inhibition of cyclin B degradation by the anaphase-promoting complex. Rsk phosphorylates and activates the Bub1 protein kinase, which may cause metaphase arrest due to inhibition of the anaphase-promoting complex (APC) by a conserved mechanism defined genetically in yeast and mammalian cells. CSF arrest in vertebrate oocytes by p90Rsk provides a potential link between the MAPK pathway and the spindle assembly checkpoint in the cell cycle.

PMID:
11730319
DOI:
10.1016/s0248-4900(01)01127-3
[Indexed for MEDLINE]

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