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Curr Microbiol. 2002 Jan;44(1):31-7.

4-Hydroxybenzoate uptake in Klebsiella planticola strain DSZ1 is driven by DeltapH.

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Dpt. Fisica Aplicada, Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, Spain.


Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 x 10-6 (cpm/OD) h-1, and a rate of 4-HBA utilisation of 0.75 mmol h-1. Aerobically the 4HBA transport system is driven by gradient of protons (DeltapH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 microM with a Vmax value of 25 nmol/ at pH 7.

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