Format

Send to

Choose Destination
Anal Chem. 2001 Nov 1;73(21):5228-31.

Characterization of protein biomarkers desorbed by MALDI from whole fungal cells.

Author information

1
Department of Chemistry and Biochemistry, University of Maryland, College Park 20742, USA. eliasi@wam.umd.edu

Abstract

In this publication, the use of ultraviolet (UV) matrix-assisted laser desorption/ionization (MALDI) time-of-fight (TOF) mass spectrometry (MS) for rapid identification and characterization of Saccharomyces cerevisiae, a fungus, is reported. S. cerevisiae is a unicellular eukaroyte that can serve as a model to study more complex organisms. We have determined that the best technique for cell wall lyses for MALDI involves the use of high concentrations of formic acid solutions. We also have shown that different fungal species exhibit different mass spectra, which can be used to distinguish them readily. Protein peaks from S. cerevisiae spectra have been tentatively identified using bioinformatics and are mainly assigned to ribosomal and mitochondrion-related proteins.

PMID:
11721923
DOI:
10.1021/ac010651t
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center