Diminished corneal angiogenesis in gelatinase A-deficient mice

FEBS Lett. 2001 Nov 16;508(2):187-90. doi: 10.1016/s0014-5793(01)02897-6.

Abstract

The goal of the present study was to define the role of gelatinase A in angiogenesis. We performed corneal micropocket assays in gelatinase A-deficient mice and their age-matched wild-type littermates. The corneal neovascular area in gelatinase A-deficient mice (0.15+/-0.14 mm(2)) was significantly less than that of wild-type littermates (0.53+/-0.35 mm(2); P<0.01). Similarly, aortic ring assays showed significant reduction of endothelial outgrowth in gelatinase A-deficient mice (0.26+/-0.14 mm(2)) as compared to wild-type littermates (0.44+/-0.06 mm(2); P<0.05). These results suggest that gelatinase A may play an important role in the regulation of corneal angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aorta / cytology
  • Aorta / drug effects
  • Cell Movement / drug effects
  • Cornea / blood supply*
  • Cornea / enzymology
  • Cornea / metabolism
  • Cornea / pathology
  • Corneal Neovascularization / enzymology*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Fibroblast Growth Factor 2 / pharmacology
  • Gene Deletion
  • Genotype
  • Immunohistochemistry
  • Matrix Metalloproteinase 2 / deficiency*
  • Matrix Metalloproteinase 2 / genetics
  • Matrix Metalloproteinase 2 / metabolism*
  • Mice
  • Mice, Knockout
  • Microscopy, Confocal
  • Neovascularization, Physiologic / drug effects

Substances

  • Fibroblast Growth Factor 2
  • Matrix Metalloproteinase 2