Abstract
A hydroxysteroid sulfotransferase (ST2A1) was identified as a form mediating neurosteroid sulfation in rat brain. The sole expression among known rat ST2A forms was indicated by brain RT-PCR. All nucleotide sequences of seven ST2A cDNA clones isolated from brain matched completely with that of hepatic ST2A1. The recombinant ST2A1 protein mediated neurosteroid sulfation. These data strongly suggest a functional role of ST2A1 as a neurosteroid sulfotransferase in rat brain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Brain / enzymology*
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Corticosterone / pharmacology
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Cytosol / enzymology
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Dehydroepiandrosterone Sulfate / metabolism
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Female
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Isoenzymes / genetics
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Isoenzymes / metabolism
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Liver / enzymology
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Male
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Phosphoadenosine Phosphosulfate / pharmacology
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Pregnenolone / pharmacology
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RNA, Messenger / biosynthesis*
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Sulfotransferases / analysis
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Sulfotransferases / biosynthesis*
Substances
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Isoenzymes
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RNA, Messenger
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Phosphoadenosine Phosphosulfate
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Dehydroepiandrosterone Sulfate
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Pregnenolone
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Sulfotransferases
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alcohol sulfotransferase
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Corticosterone