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Pflugers Arch. 2001 Nov;443(2):323-30.

Local photolysis using tapered quartz fibres.

Author information

1
Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.

Abstract

We describe a versatile, low-cost photolysis system in which sub-millisecond flashes generated by a xenon flash lamp are conveyed through a silica fibre directly onto a cell. The fibre, which is tapered to a sub-micrometre diameter and sputtered with chromium and aluminium, illuminates an area of about 1 microm2. Site and timing of photolysis can be chosen independently from other experimental parameters. As an application example we show the spatially heterogeneous distribution of Ca2+-dependent Cl- channels in olfactory receptor neurons.

PMID:
11713661
DOI:
10.1007/s004240100715
[Indexed for MEDLINE]

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