Send to

Choose Destination
Br J Dermatol. 2001 Oct;145(4):630-2.

A simple and sensitive test for field diagnosis of post kala-azar dermal leishmaniasis.

Author information

Molecular Biology Laboratory, Institute of Pathology (ICMR), Safdarjung Hospital Campus, Post Box 4909, New Delhi 110, 029, India.



Current methods for diagnosis of post kala-azar dermal leishmaniasis (PKDL) do not offer adequate sensitivity and specificity.


To develop a simple and sensitive test for field diagnosis of PKDL.


Immunochromatographic nitrocellulose strips precoated with recombinant k39 antigen were evaluated for the detection of circulating antibodies to leishmanial k39 in PKDL sera. A drop of serum applied to the strip followed by buffer led to the development of two visible bands indicating the presence of anti-k39 IgG.


The strip test was able to detect cases of PKDL with 91% sensitivity. The specificity of the test was evaluated using controls with other skin diseases, other common infections and healthy persons from endemic and non-endemic regions. Of 125 controls examined, all were negative on the strip test, indicating 100% specificity of the test.


The immunochromatographic nitrocellulose strips provide a non-invasive, rapid and accurate method for diagnosing PKDL.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center