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DNA Seq. 2001;11(6):515-8.

Cloning of a cDNA encoding an isoform of human protein phosphatase inhibitor 2 from vascularized breast tumor.

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Laboratory for Surgical Research, Children's Hospital, Department of Surgery, Harvard Medical School, 320 Longwood Ave., Boston, MA 02115, USA.


Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3'-end RACE and 5'-end RACE. Analysis of the full-length cDNA of this gene reveals that this cDNA encodes an open reading frame of 615 bp, which is highly homologous to human protein phosphatase inhibitor-2, with 92% identity at the nucleotide level, and 89% identity at amino acid level. The results of this study suggest that this novel isoform of human protein phosphatase inhibitor-2 (nPPI-2) may be involved in the angiogenic switch during breast tumor development.

[Indexed for MEDLINE]

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