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Clin Chim Acta. 2001 Nov;313(1-2):139-42.

About the possible origin and mechanism of circulating DNA apoptosis and active DNA release.

Author information

1
Lab de Biochimie et Physiologie Vegetale, Faculté des Sciences, Pavillon des Isotopes, bvd d'Yvoy 20, 1211, Geneva, Switzerland.

Abstract

BACKGROUND:

In addition to cell lysis, apoptosis has been advanced as the origin of circulating DNA on the basis of several observations. Plasma or serum DNA often presents a ladder pattern reminiscent of that displayed by apoptotic cells when subjected to electrophoresis. However, the phenomenon of active release of DNA from cells might also be expected to result in a ladder pattern on electrophoresis. Non-dividing cells, such as lymphocytes, frog auricles and cultured cell lines including HL-60, spontaneously release a nucleoprotein complex within a homeostatic system in which newly synthesized DNA is preferentially released.

CONCLUSION:

In relation to DNA synthesis, the phenomenon of extracellular DNA in different culture conditions favors apoptosis or spontaneous active DNA release.

PMID:
11694251
[Indexed for MEDLINE]

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