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Genesis. 2001 Sep;31(1):6-10.

Efficient FLP recombination in mouse ES cells and oocytes.

Author information

1
Gene Expression Program, EMBL, Heidelberg, Germany.

Abstract

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

PMID:
11668672
[Indexed for MEDLINE]

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