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EMBO Rep. 2001 Nov;2(11):1047-51. Epub 2001 Oct 17.

The cystic fibrosis transmembrane conductance regulator (CFTR) inhibits ENaC through an increase in the intracellular Cl- concentration.

Author information

1
School of Biomedical Sciences, Department of Physiology and Pharmacology, University of Queensland, St Lucia, Queensland 4072, Brisbane, Australia.

Abstract

Activation of the CFTR Cl- channel inhibits epithelial Na+ channels (ENaC), according to studies on epithelial cells and overexpressing recombinant cells. Here we demonstrate that ENaC is inhibited during stimulation of the cystic fibrosis transmembrance conductance regulator (CFTR) in Xenopus oocytes, independent of the experimental set-up and the magnitude of the whole-cell current. Inhibition of ENaC is augmented at higher CFTR Cl- currents. Similar to CFTR, ClC-0 Cl- currents also inhibit ENaC, as well as high extracellular Na+ and Cl- in partially permeabilized oocytes. Thus, inhibition of ENaC is not specific to CFTR and seems to be mediated by Cl-.

PMID:
11606421
PMCID:
PMC1084131
DOI:
10.1093/embo-reports/kve232
[Indexed for MEDLINE]
Free PMC Article

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