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Curr Opin Biotechnol. 2001 Oct;12(5):503-9.

Mutagenesis in the post genomics era: tools for generating insertional mutations in the lactic acid bacteria.

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Department of Viticulture and Enology, University of California, 1 Shields Avenue, Davis, CA 95616-8749, USA.


The increasing availability of whole genome sequences has increased the demand for effective tools to generate insertional mutations in the lactic acid bacteria (LAB). Several novel approaches, such as shuttle-, transposome- and intron-based mutagenesis methods, are possible additions to the existing repertoire of transposon- and recombination-based tools available for mutagenesis of LAB.

[Indexed for MEDLINE]

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