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Curr Opin Biotechnol. 2001 Oct;12(5):473-80.

Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes.

Author information

1
Gesellschaft für Biotechnologische Forschung mbH (GBF), German Research Institute for Biotechnology, RDIF/Epigenetic Regulation, D-38124 Braunschweig, FRG, Mascheroder Weg 1, Braunschweig, Germany. aba@gbf.de

Abstract

Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.

PMID:
11604323
DOI:
10.1016/s0958-1669(00)00248-2
[Indexed for MEDLINE]

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