Gene expression monitoring in soils by mRNA analysis and gene lux fusions

Curr Opin Biotechnol. 2001 Oct;12(5):455-60. doi: 10.1016/s0958-1669(00)00245-7.

Abstract

Two methods recently developed to monitor the gene expression of microbial communities in soil are the extraction and detection of messenger RNA from soil microorganisms and the construction and use of lux-based bioreporter strains. The goal of these approaches is to assess microbial activity in natural and impacted soil environments.

Publication types

  • Review

MeSH terms

  • Artificial Gene Fusion
  • Gene Expression Profiling / methods*
  • Genes, Bacterial
  • Genes, Reporter*
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / metabolism
  • RNA, Messenger / analysis*
  • Recombinant Fusion Proteins / metabolism
  • Soil Microbiology*

Substances

  • Luminescent Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins