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Curr Opin Biotechnol. 2001 Oct;12(5):439-45.

Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads.

Author information

1
Department of Microbiology, Colorado State University, Fort Collins, CO 80523, USA. herbert.schweizer@colostate.edu

Abstract

Improved tools for Pseudomonas research include small, broad-host-range vectors that allow regulated expression from the lac operon and T7 promoters whose biology is well understood and adaptable to many bacteria. To facilitate studies on gene regulation, tracking and monitoring of bacteria in diverse environments, and the construction of biosensors, various reporter genes with versatile assay formats have been developed that can be delivered on plasmid, transposon and integration-proficient vectors.

PMID:
11604317
DOI:
10.1016/s0958-1669(00)00242-1
[Indexed for MEDLINE]

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