Differential regulation of interleukin-12 and interleukin-10 by heat shock response in murine peritoneal macrophages

Biochem Biophys Res Commun. 2001 Oct 12;287(5):1041-4. doi: 10.1006/bbrc.2001.5704.

Abstract

Heat shock response is a conserved stress response and has been shown to have anti-inflammatory effects. We investigated the effect of heat shock response on LPS-induced production of IL-12 and IL-10, which are two important cytokines playing contradictory roles in regulation of immune response, by murine peritoneal macrophages. The data showed that induction of heat shock response strongly suppressed LPS-induced production of IL-12 while augmented that of IL-10, suggesting the pleiotropic effects of heat shock response on immune regulatory gene expression. Also, the novel observation on up-regulation of IL-10 by heat shock response adds to the mechanism by which heat shock response exerts its anti-inflammatory effects.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Gene Expression Regulation
  • HSP70 Heat-Shock Proteins / biosynthesis
  • HSP70 Heat-Shock Proteins / genetics
  • Heat-Shock Response*
  • Inflammation
  • Interleukin-10 / biosynthesis*
  • Interleukin-12 / biosynthesis*
  • Lipopolysaccharides / immunology
  • Macrophages, Peritoneal / immunology*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • RNA, Messenger / biosynthesis

Substances

  • HSP70 Heat-Shock Proteins
  • Lipopolysaccharides
  • RNA, Messenger
  • Interleukin-10
  • Interleukin-12