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Int J Radiat Biol. 2001 Aug;77(8):901-8.

FISH examination of lymphocytes from Mayak workers for assessment of translocation induction rate under chronic radiation exposures.

Author information

1
Clinical Department, Branch N1, Institute of Biophysics, Ozyorsk's shosse 19, Ozyorsk, Chelyabinsk 456780, Russian Federation.

Abstract

PURPOSE:

To estimate the translocation-induction rate under chronic exposure conditions by measuring chromosome aberration frequencies in lymphocytes from Mayak nuclear workers using fluorescence in situ hybridization (FISH).

MATERIALS AND METHODS:

Lymphocytes were examined from 27 nuclear workers at the Mayak Production Association and two control individuals using FISH with probes for chromosomes 1, 2 and 4. Official doses derived from worker film-badge records varied from 0 to 8.50 Gy.

RESULTS:

The mean (+/-SD) genome-equivalent translocation frequency (F(G)) was 2.30 (+/-0.75)% in the zero-dose group (n = 7), and Poisson regression analysis provided the best-fit equation of F(G)(%) = 2.96(+/-0.39) + 0.69(+/-0.14)D + 0.12(+/-0.05)A, where D is the film-badge-derived dose (Gy), and A is age centred at 67 years. The induction rate would increase to nearly 1% Gy(-1) if the radiation dose to bone marrow, one of the major organs for lymphocytes and where their precursor cells reside, is considered.

CONCLUSION:

The estimated induction rate in vivo appeared substantially smaller than linear coefficients estimated from various in vitro studies.

PMID:
11571024
DOI:
10.1080/09553000110063386
[Indexed for MEDLINE]

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