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Mol Cells. 2001 Aug 31;12(1):91-6.

Molecular cloning and functional expression of bovine brain GABA transaminase.

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Department of Genetic Engineering, Hallym University, Chunchon, Korea.


We isolated a cDNA that encodes the bovine brain gamma-aminobutyrate transaminase (GABA-T; EC from the lambda gt 11 cDNA library, which showed a high degree of sequence similarity to the corresponding enzymes from various sources. Northern blot analysis revealed two differentially expressed GABA-T transcripts of approximately 2.0 and 6.0 kb in the bovine tissues. Southern blot analysis indicates that the two GABA-T transcripts are encoded in a greater-than 10-kb, single-copy gene. Bovine GABA-T cDNA was expressed in E. coli using the pGEX bacterial- expression vector system. The overexpressed GABA-T was enzymatically active after purification, and it had very similar kinetic parameters when compared with those of other mammalian GABA-Ts.

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