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Ann Intern Med. 2001 Sep 18;135(6):434-8.

Fatal pseudomembranous colitis associated with a variant clostridium difficile strain not detected by toxin A immunoassay.

Author information

1
Medical Service, Veterans Affairs Chicago Health Care System-Lakeside, 333 East Huron Street, Chicago, IL 60611, USA.

Abstract

BACKGROUND:

Many clinical laboratories use toxin A immunoassays to test for Clostridium difficile.

OBJECTIVE:

To describe the clinical course of a patient infected with a toxin variant strain of C. difficile that was not detected by toxin A immunoassay; to genetically characterize this strain; and to estimate the number of laboratories that use only toxin A immunoassays.

DESIGN:

Case report, molecular investigation, and laboratory survey.

SETTING:

Tertiary care hospital in Chicago, Illinois.

PATIENT:

An 86-year-old man.

MEASUREMENTS:

Restriction endonuclease analysis, polymerase chain reaction, and survey of regional clinical laboratories.

RESULTS:

An elderly hospitalized man died of advanced pseudomembranous colitis. Four stool specimens submitted over a 2-month period had tested negative on toxin A immunoassay, but a strain of C. difficile with a 1.8-kb deletion of the toxin A gene was recovered from each specimen. This strain, identified as restriction endonuclease analysis type CF4, is closely related to a widely disseminated variant, toxinotype VIII. Toxin A immunoassay was the only test being performed for detection of C. difficile at 31 of 67 (46%) regional clinical laboratories.

CONCLUSIONS:

Toxin A variant strains of C. difficile cause serious disease and are undetectable in clinical laboratories that use only toxin A immunoassays for C. difficile testing.

[Indexed for MEDLINE]

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