Format

Send to

Choose Destination
Methods. 2001 Sep;25(1):78-86.

Single-molecule fluorescence resonance energy transfer.

Author information

1
Department of Physics, Center for Biophysics and Computational Biology, Urbana 61801, USA. tjha@uiuc.edu

Abstract

Fluorescent resonance energy transfer (FRET) is a powerful technique for studying conformational distribution and dynamics of biological molecules. Some conformational changes are difficult to synchronize or too rare to detect using ensemble FRET. FRET, detected at the single-molecule level, opens up new opportunities to probe the detailed kinetics of structural changes without the need for synchronization. Here, we discuss practical considerations for its implementation including experimental apparatus, fluorescent probe selection, surface immobilization, single-molecule FRET analysis schemes, and interpretation.

PMID:
11558999
DOI:
10.1006/meth.2001.1217
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center