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Biochim Biophys Acta. 2001 Oct 1;1514(2):170-6.

Imaging erythrocytes under physiological conditions by atomic force microscopy.

Author information

1
Department of Chemical Engineering and Chemical Technology, Imperial College of Science, Technology and Medicine, London, UK.

Abstract

Since its invention in the mid 1980s atomic force microscopy has revolutionised the way in which surfaces can be imaged. Close to atomic resolution has been achieved for some materials and numerous images of molecules on surfaces have been recorded. Atomic force microscopy has also been of benefit to biology where protein molecules on surfaces have been studied and even whole cells have been investigated. Here we report a study of red blood cells which have been imaged in a physiological medium. At high resolution, the underlying cytoskeleton of the blood cell has been resolved and flaws in the cytoskeleton structure may be observed. Comparison of the normal 'doughnut' shaped cells with swollen cells has been undertaken. Differences in both the global properties of the cells and in the local features in cytoskeleton structure have been observed.

PMID:
11557018
DOI:
10.1016/s0005-2736(01)00365-0
[Indexed for MEDLINE]
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