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Curr Opin Biotechnol. 2001 Aug;12(4):419-25.

Using proteins in their natural environment: potential and limitations of microbial whole-cell hydroxylations in applied biocatalysis.

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Institute of Biotechnology, ETH Hönggerberg, HPT, CH 8093, Zürich, Switzerland.


The unique catalytic properties of oxygenases (the regio-specific and/or enantio-specific hydroxylation of non-activated carbons) are of undisputed biosynthetic value. Factors that govern the economics of their industrial use include a low k(cat), a frequently decreased k(cat) in recombinant strains, limiting oxygen transfer rates in bioreactors, product inhibition, and the demanding discovery (screening) process.

[Indexed for MEDLINE]

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