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Microbiology. 2001 Sep;147(Pt 9):2399-408.

Identification of the heat-shock sigma factor RpoH and a second RpoH-like protein in Sinorhizobium meliloti.

Author information

1
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA. voke@pitt.edu

Abstract

Hybridization to a PCR product derived from conserved sigma-factor sequences led to the identification of two Sinorhizobium meliloti DNA segments that display significant sequence similarity to the family of rpoH genes encoding the sigma(32) (RpoH) heat-shock transcription factors. The first gene, rpoH1, complements an Escherichia coli rpoH mutation. Cells containing an rpoH1 mutation are impaired in growth at 37 degrees C under free-living conditions and are defective in nitrogen fixation during symbiosis with alfalfa. A plasmid-borne rpoH1-gusA fusion increases in expression upon entry of the culture into the stationary phase of growth. The second gene, designated rpoH2, is 42% identical to the S. meliloti rpoH1 gene. Cells containing an rpoH2 mutation have no apparent phenotype under free-living conditions or during symbiosis with the host plant alfalfa. An rpoH2-gusA fusion increases in expression during the stationary phase of growth. The presence of two rpoH-like sequences in S. meliloti is reminiscent of the situation in Bradyrhizobium japonicum, which has three rpoH genes.

PMID:
11535780
DOI:
10.1099/00221287-147-9-2399
[Indexed for MEDLINE]

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