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Biotechniques. 2001 Aug;31(2):322-3, 326-8.

Novel vectors for co-expression of two proteins in E. coli.

Author information

1
La Jolla Cancer Research Center, La Jolla, CA, USA.

Abstract

Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows for an efficient expression and purification of large amounts of protein-protein complexes formed in bacterial cells. We have utilized this new system to express and isolate a stable complex of two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) and mitogen-activated proteins kinase Erk2. This approach is useful for biochemical and structural studies of protein-protein interactions.

PMID:
11515368
DOI:
10.2144/01312st03
[Indexed for MEDLINE]
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