Format

Send to

Choose Destination
See comment in PubMed Commons below
J Biol Chem. 2001 Oct 26;276(43):40263-7. Epub 2001 Aug 20.

Regulation of heat shock transcription factor 1 by stress-induced SUMO-1 modification.

Author information

  • 1Department of Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA.

Erratum in

  • J Biol Chem 2002 Jul 19;277(29):26708. Goodson M [corrected to Goodson ML].

Abstract

Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress HSF1 acquires DNA binding ability and localizes to nuclear stress granules, but the molecular mechanisms that mediate these events are not understood. We report that HSF1 undergoes stress-induced modification at lysine 298 by the small ubiquitin-related protein called SUMO-1. Antibodies against SUMO-1 supershift the HSF1 DNA-binding complex, and modification of HSF1 in a reconstituted SUMO-1 reaction system causes conversion of HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of lysine 298. Mutation of lysine 298 also results in a significant decrease in stress-induced transcriptional activity of HSF1 in vivo. This work implicates SUMO-1 modification as an important modulator of HSF1 function in response to stress.

PMID:
11514557
DOI:
10.1074/jbc.M104714200
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Support Center