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Pharmazie. 2001 Jul;56(7):536-41.

Chiral separation of verapamil and some of its metabolites by HPLC and CE.

Author information

1
Department of Analytical Chemistry, Slovak Technical University, Bratislava, Slovakia. branstet@cvt.suba.sk

Abstract

HPLC and CE assays were developed for chiral separations of verapamil and its metabolites in serum samples. Three chiral HPLC columns (Chiralcel OJ, Chiralpak AD and Chiralcel OD-R) were tested in normal and reverse-phase modes. All HPLC analyses were performed with fluorescence detection at 276 and 310 nm. CE was realized using CM-beta-CD as a chiral selector for the enantiomeric analysis. The results of HPLC and CE studies were compared and the possibilities for the applications in therapeutic drug monitoring were discussed.

PMID:
11487971
[Indexed for MEDLINE]

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